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Old 04-25-2012, 04:40 AM   #16
nickloman
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Location: Birmingham, UK

Join Date: Jul 2009
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Quote:
Originally Posted by pmiguel View Post
That is, it looks to me from the chart that the MiSeq bin of bases with a quality score of 20 (1 error in 100) actually had 1 error in 1000. Indicating that they really were Q30 bases, rather than Q20 bases?
That's right, but bear in mind there were very few bases with that quality in the dataset (c.f. top panel of Figure 1). I tried to represent this with the area of the point, but I'm not sure I did a very good job getting the dynamic range across.
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