Dear All,
I am pretty new for Illumina Miseq platform.
The results I got are demultiplexed paired end reads. It has been quality filtered based on the quality score 25.
What I have done is to remove both the forward and reverse primer using QIIME and merge the reads, then quality-filtering (-fastq_maxee 1.0 ) the reads using usearch. However, when I pick up OTUs, there are too many OTUs.
I am wondering if anybody who did the similar work in the past can give me some suggestions.
Best reagards,
Zhigang
I am pretty new for Illumina Miseq platform.
The results I got are demultiplexed paired end reads. It has been quality filtered based on the quality score 25.
What I have done is to remove both the forward and reverse primer using QIIME and merge the reads, then quality-filtering (-fastq_maxee 1.0 ) the reads using usearch. However, when I pick up OTUs, there are too many OTUs.
I am wondering if anybody who did the similar work in the past can give me some suggestions.
Best reagards,
Zhigang
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