Hi,
I am trying to enrich my pair-end library. I made two libraries 300 bp and 600 bp. At the end PCR step, I get only 26 ng/ul and 15ng/ul for the libraries. This was estimated by nanodrop. I used 10 PCR cycles. How can I get more library. Do I have problems with my adapters that they are not ligated properly. Any help is really appreciated.
I am trying to enrich my pair-end library. I made two libraries 300 bp and 600 bp. At the end PCR step, I get only 26 ng/ul and 15ng/ul for the libraries. This was estimated by nanodrop. I used 10 PCR cycles. How can I get more library. Do I have problems with my adapters that they are not ligated properly. Any help is really appreciated.
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