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Old 12-22-2015, 12:46 AM   #3
Location: China

Join Date: Oct 2010
Posts: 55

Thanks a lot @bowhan
Yes. I have do the size selection. But we still can't fully sequence the entire lengths of long transcript. Trunction of sequence could occur form RNA defradation,mechanical shearing of the sample, incomplete PCR amplification, or loading bias of the sequencer.
So, IS there any software can be uesed to complete the sequence with illumina short reads by extending the ending of the sequences?
Thanks a lot again.
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