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Old 12-22-2015, 10:28 PM   #6
huan
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Location: China

Join Date: Oct 2010
Posts: 55
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Thanks a lot for your [email protected] @nucacidhunter.
That's quite a great idea to ensure the integrity of 5' end by library building.
But what I want is to deal with it by analysis method(software), such as extending the end of the transcript by assemble using short reads considering the alternative splicing condition. I am not sure is there any software or workflow could achieve this.
Any suggestion will be appreciated!
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