Hi,
Forgive me if this is naive, but I'm still newish to thinking about sequencing. I've begun working with the DADA2 software and the tutorial clearly states that primers should be trimmed because they are non-biological nucleotides.
I'm not arguing against it, but I don't fully understand it. If the primer matches a sequence of DNA, then shouldn't it be just as legitimate a sequence as the sequence derived from the sequencing process?
Any thoughts?
Thanks,
Steve
Forgive me if this is naive, but I'm still newish to thinking about sequencing. I've begun working with the DADA2 software and the tutorial clearly states that primers should be trimmed because they are non-biological nucleotides.
I'm not arguing against it, but I don't fully understand it. If the primer matches a sequence of DNA, then shouldn't it be just as legitimate a sequence as the sequence derived from the sequencing process?
Any thoughts?
Thanks,
Steve
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