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Old 03-18-2016, 08:51 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
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Possible causes:
1- Input DNA
2- Reagent issues
3- Reaction set up practices
4- Thermocycler issues (can check the temperature with a probe or a thermometer)

To narrow down the cause I would set up reactions using another DNA that has worked fine before or a clean commercial DNA with the same reagents that were used for failed reactions. It will be useful to post electropherogram of sample before and after failed tagmentation.
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