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Old 03-21-2016, 03:11 PM   #4
Location: United States

Join Date: May 2014
Posts: 40

Nextera XT requires input DNA of fairly low concentration (0.2ng/uL). What platforms are you using to measure your samples' concentration? Are you using QIAxcel readings to make your dilutions before tagmentation?

As nucacidhunter mentioned, an electropherogram will be quite helpful. When you say that there is no tagmentation occurring do you mean that the traces from before and after look identical?
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