Hi all,
I am having difficulty in assessing how many million reads are required to optimally capture a transcriptome ?.
I have typically 13 million reads from a Miseq run for 7 samples (1.85 million paired end reads/sample ). Is this number sufficient for carrying out typical RNA-seq analysis using tophat/cufflinks pipeline ? If not what is the optimal number of reads required ?
Thanks!
Regards,
SKM
I am having difficulty in assessing how many million reads are required to optimally capture a transcriptome ?.
I have typically 13 million reads from a Miseq run for 7 samples (1.85 million paired end reads/sample ). Is this number sufficient for carrying out typical RNA-seq analysis using tophat/cufflinks pipeline ? If not what is the optimal number of reads required ?
Thanks!
Regards,
SKM
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