SEQanswers - View Single Post - High-Throughput DNA Shearing

EPICENTRE · 03-04-2009, 08:20 AM

We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?

03-04-2009, 08:20 AM	#2
EPICENTRE Registered Vendor Location: Madison Join Date: Mar 2009 Posts: 5	We are working on a way to fragment and add adaptors using a transposon-based method. Our fragments tend to smear in the 150-2000 bp range. How critical is it for you to be in the 150-200 bp range? We can gel purify, but would like to eliminate this step if possible. Which adaptors do you need to add?