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Originally posted by GenoMax
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IMHO this is not necessary; Lane X should look good, that's more important :-) We do this from time to time with chipseq data. -
If there was a control lane defined then there should be no need to go messing about with matrix or phasing settings (assuming the control lane looked OK).Originally posted by victorsor View Post
If you've still got no clusters then the only remaining remedy would be to run the bclToFastq with the --no-eamss option to see if the qualities temporarily dip and then recover.Comment
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Thank you all. We will try to do your suggestions when run finish. I hope that not all data go to waste...Comment
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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