View Single Post
Old 04-03-2013, 08:11 AM   #4
Amative
Member
 
Location: USA

Join Date: Dec 2011
Posts: 45
Default

Thanks swbarnes2 & rboettcher,

@swbarnes2
  • I tried to blast the first ten reads from one of the samples I have, blast results were not that good. I tried to align against the available sequences of the two of the top blast hits. Same low alignment rate.
  • I checked for adapters, sequences are already trimmed.

@rboettcher
Yes, I am aligning against the transcriptome sequences.
Amative is offline   Reply With Quote