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Old 04-03-2013, 08:11 AM   #4
Location: USA

Join Date: Dec 2011
Posts: 45

Thanks swbarnes2 & rboettcher,

  • I tried to blast the first ten reads from one of the samples I have, blast results were not that good. I tried to align against the available sequences of the two of the top blast hits. Same low alignment rate.
  • I checked for adapters, sequences are already trimmed.

Yes, I am aligning against the transcriptome sequences.
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