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Old 01-08-2014, 12:58 AM   #2
Location: Berlin

Join Date: Oct 2010
Posts: 71

Hi lre1234,

If you are looking at a pool of samples this might actually be a true thing, but I would check for repetitive or very similar sequences on the other side of the fusion to check if you are looking at a mapping artifact. It could be that mate 1 is mapping uniquely and mate 2 has multiple hits.
rboettcher is offline   Reply With Quote