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Old 08-28-2014, 08:53 PM   #15
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Originally Posted by eastasiasnow View Post
yeah, pooling biological replicate samples will lose group variance. but could I use this design to do the following analysis? do people accept this design when I apply it in my paper? if so, what kind of tools can do this?

thank you very much.
For differential expression analysis, I wouldn't. That design would have a lot of trouble getting published. For almost the same price you can sequence biological replicates that have been individually tagged and get results that are far more biologically relevant.
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