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Old 05-19-2015, 07:13 AM   #2
Junior Member
Location: Montreal, Canada

Join Date: Nov 2014
Posts: 4

Hi Amelia,
I found this question and I am wondering if you had any luck making adjustments to make longer libraries with Nextera? I prepared libraries with Nextera last year and also got shorter fragment sizes than ideal (200-250 bp) when I would like 300-400 bp. I am wondering if it would be best to use a higher concentration of DNA or lower concentration of enzyme?
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