SEQanswers - View Single Post - Nextera Kit -- DNA fragments too small

Jfly7 · 05-19-2015, 07:13 AM

Hi Amelia,
I found this question and I am wondering if you had any luck making adjustments to make longer libraries with Nextera? I prepared libraries with Nextera last year and also got shorter fragment sizes than ideal (200-250 bp) when I would like 300-400 bp. I am wondering if it would be best to use a higher concentration of DNA or lower concentration of enzyme?

05-19-2015, 07:13 AM	#2
Jfly7 Junior Member Location: Montreal, Canada Join Date: Nov 2014 Posts: 4	Hi Amelia, I found this question and I am wondering if you had any luck making adjustments to make longer libraries with Nextera? I prepared libraries with Nextera last year and also got shorter fragment sizes than ideal (200-250 bp) when I would like 300-400 bp. I am wondering if it would be best to use a higher concentration of DNA or lower concentration of enzyme?