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Old 05-19-2015, 08:02 AM   #3
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Location: USA

Join Date: Apr 2009
Posts: 482

Single cell RNA-Seq libraries with an input of 1 ng or less of cDNA give Nextera fragment sizes of over 500 bp.

Note with the HiSeq 3000/4000 all libraries have to be less than 500 bp so you might want to reconsider whether you want larger fragments.
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