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Old 05-19-2015, 09:17 AM   #7
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Location: Purdue University, West Lafayette, Indiana

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Originally Posted by NextGenSeq View Post
What I find most annoying about Nextera is the insertion bias.
Every Nextera I've ever seen shows unusual GC bias in the first 15 basepairs (see attached).
Nextera is Tn5. When you recruit a natural protein to do a job for you, you recruit its natural proclivities as well. Sure they have engineered it a little, but not enough to completely remove its insertion site preferences.

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