Thread: Nextera Kit -- DNA fragments too small
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Old 05-22-2015, 07:37 AM   #9
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Quote:
Originally Posted by nucacidhunter View Post
An interesting patent by Agilent was recently published about role of bivalent cations on Nextera activity.
http://patft1.uspto.gov/netacgi/nph-...S=PN/9,005,935
Very interesting!
Does Agilent actually sell this Vibhar Tn kit?
So it is likely that including Mn in Nextera reactions would reduce the site specificity. Interesting...

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Phillip
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