SEQanswers - View Single Post - Nextera Kit -- DNA fragments too small

Simone78 · 05-22-2015, 09:48 AM

Interesting! When using our home-made Tn5 the tagmentation was actually INHIBITED by MnCl2 and worked only with MgCl2 (with 5 mM final being the optimal conc, while higher conc had a deleterious effect).
Thanks for sharing,
Simone

05-22-2015, 09:48 AM	#10
Simone78 Senior Member Location: Basel (Switzerland) Join Date: Oct 2010 Posts: 208	Interesting! When using our home-made Tn5 the tagmentation was actually INHIBITED by MnCl2 and worked only with MgCl2 (with 5 mM final being the optimal conc, while higher conc had a deleterious effect). Thanks for sharing, Simone