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Old 05-22-2015, 09:48 AM   #10
Senior Member
Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 208

Interesting! When using our home-made Tn5 the tagmentation was actually INHIBITED by MnCl2 and worked only with MgCl2 (with 5 mM final being the optimal conc, while higher conc had a deleterious effect).
Thanks for sharing,
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