SEQanswers - View Single Post - On-Plate ATAC-seq

krutipedali · 02-28-2017, 05:16 AM

No, those libraries haven`t been sent for sequencing. Instead we have produced another samples. The thing is they are the way too overtagmented, so go down with incubation time(even to 5-10 minutes) or try removing permeabilization step.

02-28-2017, 05:16 AM	#3
krutipedali Junior Member Location: Norway, Bergen Join Date: Nov 2016 Posts: 3	No, those libraries haven`t been sent for sequencing. Instead we have produced another samples. The thing is they are the way too overtagmented, so go down with incubation time(even to 5-10 minutes) or try removing permeabilization step.