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Old 03-07-2017, 09:20 AM   #2
GenoMax
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Location: East Coast USA

Join Date: Feb 2008
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Use BBMap to estimate insert sizes. There are two methods described here. That estimate of 600 bp is clearly wrong since you would not have been able to merge the R1/R2 reads otherwise.

2x250 is maximum supported length on HiSeq 2500 and 2 x 300 on MiSeq. One can't get longer sequencing lengths on currently available Illumina sequencing kits. One could run asymmetric runs (e.g. 1 x 600 bp) but that is not generally recommended due to drops in quality you are bound to experience towards the end of such runs.

Last edited by GenoMax; 03-07-2017 at 09:25 AM.
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