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SDPA_Pet · 03-07-2017, 09:32 AM

Hi GenoMax,

Yes, I know the bioinformatic tools BBMAP. According to their report, it says they determine the size of library using Agilent 2100 Bioanalyzer. I have never used a Bioanalyzer. I would guess it is kind of instrument that can do physical measurement (not a bioinformatic tool). Do you suggest that their reports or measurements are wrong. I should use bioinformatic tools to check it? Is it common that bioanalyzer gives you a wrong number?

So, I am correct, right? To join 2X150 bp, most of inserts should be less than 300bp.

03-07-2017, 09:32 AM	#3
SDPA_Pet Senior Member Location: US Join Date: Apr 2013 Posts: 222	Hi GenoMax, Yes, I know the bioinformatic tools BBMAP. According to their report, it says they determine the size of library using Agilent 2100 Bioanalyzer. I have never used a Bioanalyzer. I would guess it is kind of instrument that can do physical measurement (not a bioinformatic tool). Do you suggest that their reports or measurements are wrong. I should use bioinformatic tools to check it? Is it common that bioanalyzer gives you a wrong number? So, I am correct, right? To join 2X150 bp, most of inserts should be less than 300bp.