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Old 03-08-2017, 07:25 AM   #15
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Location: Cambridge

Join Date: Sep 2010
Posts: 116
Lightbulb Clustering = Bridge Amplification (for pre ex amp).

Clustering means bridge amplification for pre ExAmp (non-patterned flowcells) - in situ PCR on the flow cell surface oligos lawn. Has similar rukes/laws to a regular PCR, only the product stays in situ, forming a forest from DNA strands.

For ExAmp Chemistry (patterned flowcells) - Clustering means cluster formation using Isothermal Amplification.
(In theory only on the occupied nanowell, in practice, especially at low loading concentrations a few neighbours may join in too...).

Have a read about ExAmp & Hiseq4000:
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