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Old 04-05-2017, 09:07 PM   #1
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Location: australia

Join Date: Apr 2017
Posts: 3
Default Exogenous spike for RNA seq

Hi all... help! New to all this next generation sequencing stuff. I need to add an exogenous spike (have used c.elegans 54 for RT PCR) for serum samples for next generation sequencing. Cant spike in too much or not enough! I cant find any papers on how much spike to use (though i have learnt what a femtamole is!Lol) Can anyone help?
Thanks in advance!
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