SEQanswers - View Single Post

cmbetts · 04-06-2017, 10:13 AM

The most commonly used exogenous spike in for RNA-Seq is the ERCCs sold through Thermo. Their user manuals have guidelines for adding the appropriate amount to different sample types to get the intended 1-5% of reads.

04-06-2017, 10:13 AM	#2
cmbetts Senior Member Location: Bay Area Join Date: Jun 2012 Posts: 120	The most commonly used exogenous spike in for RNA-Seq is the ERCCs sold through Thermo. Their user manuals have guidelines for adding the appropriate amount to different sample types to get the intended 1-5% of reads.