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Old 04-06-2017, 05:10 PM   #3
veryconfused
Junior Member
 
Location: australia

Join Date: Apr 2017
Posts: 3
Default ERCC won't help :(

Thanks... i did look into the ERCC spike and spoke to the sequencing peeps and they mentioned ERCC spike ins were more to determine technical variations. I need an exogenous spike as i have no idea what my samples are going to do! I'm looking at RNA levels in cardiac surgery patients -so there are no "normal" or "healthy" groups i am able to use as a comparison for normal micro RNA levels... i have found a couple of papers that have used ath-miR-159a but, their methods aren't terribly clear and the corresponding author isn't answering my emails
Its proving a difficult task! But thanks for your input - i appreciate your reply
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