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Old 04-08-2017, 06:25 AM   #7
GenoMax
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Location: East Coast USA

Join Date: Feb 2008
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In your case it sounds like all you want/need is a technical spike to say that the reads for the spike are similarr in both samples leading to an assumption that the rest of the sample behaved identically (as far as the conversion to DNA/Sequencing went).

Someone will have to comment on what/how much to add but be aware that at the amounts we are talking about you will never get an identical number of reads in two samples for the spike.

If you have a clear signal from the tests so far it should be replicable, if it is real, across more samples. Having millions of reads should not mask it as long as you follow some established normalization procedure for your counts.
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