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Old 04-10-2017, 08:20 AM   #2
thermophile
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Location: CT

Join Date: Apr 2015
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You could do direct amplicon sequencing, the way that most people are doing 16s for bacterial communities. (see Kozich 2013 or Caporasso 2012). Depending on how many samples you have, you may want to design your own primers or use a 2-step method (Lange 2014)

I run a core facility that specialized in amplicon sequencing, you can check out my website for some documentation and visualizations. mars.uconn.edu
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Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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