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Old 09-14-2017, 06:28 AM   #2
huguesparri
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Location: Montpellier (France)

Join Date: May 2008
Posts: 93
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Hello,

In my opinion, the most sensitive reagent in the kit is the ligation mix.
But it's hard to say if it suffered from standing at RT without testing it.
if you have any doubt, you can try to substitute it with NEB quick ligation kit or T4 DNA ligase.
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