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Old 03-09-2018, 05:08 AM   #1
thiNGS
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Location: Netherlands

Join Date: Sep 2014
Posts: 24
Talking bacterial RNAseq using TruSeq Lib prep

Hi all. I am in the process of starting RNAseq from bacterial samples and I am trying to identify the best strategy to do it. Given that bacterial RNA is not poly-adenylated, a random hexamer-based system is essential. Has anyone used the TruSeq stranded library prep kit for bacterial RNA? Or do you have a good kit to recommend? The samples will be run on the MiSeq or NextSeq systems. Any advice to guide me through the magical world of RNAseq will be much appreciated
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