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Old 02-27-2019, 11:03 PM   #1
Junior Member
Location: delhi

Join Date: Feb 2019
Posts: 4
Question De novo assembly

hello everyone,

I have recently started working in a lab and have been assigned the following task.

AIM:To assemble the given sequences as one consensus for transformation to be even more specific. Looking for regions around trnA, trnI, 23s and 16s.

Data I have:
1) chloroplast genome sequence of an algae along with its circular map.

2) Sequences of the Primers used. Three sets of primers have been used (forward and reverse) along with trnaI and trnaA (both forward and reverse).
The sequences are in ".ab1" format.

Please let me know how will you approach this problem.

PS: No other files or data has been handed over to me. Its someone else's work and that person has left.
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