SEQanswers - View Single Post - ATAC-seq library sequencing

luc · 03-12-2019, 01:19 PM

Hi Olga,

your math seems correct. Six samples to sequence about 50 million library molecules each. Buenrostro et al. generally refer to paired-reads in their papers.
But you can certainly pool more libraries, analyze the data of the first lane, and then sequence additional lanes if needed.

03-12-2019, 01:19 PM	#2
luc Senior Member Location: US Join Date: Dec 2010 Posts: 453	Hi Olga, your math seems correct. Six samples to sequence about 50 million library molecules each. Buenrostro et al. generally refer to paired-reads in their papers. But you can certainly pool more libraries, analyze the data of the first lane, and then sequence additional lanes if needed.