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Old 04-05-2019, 02:56 AM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

Generally smaller inserts would result in more uniform coverage of genes so for counting applications short inserts are preferred and can be sequenced only 70 cycles. For other applications such as transcriptome assembly larger fragments and high number of sequencing cycles are more efficient. Some kits such as NEBNext Ultra II enable better customisation of insert size ranging from 150bp-1kb.
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