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Old 04-05-2019, 08:00 AM   #1
AndrewP
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Location: Boston

Join Date: Mar 2019
Posts: 11
Default Need high number of short cDNA fragments

Hi all,

My application requires short cDNA fragments (up to 500 bp) with a defined 5' tag. I don't have the option to fragment RNA before reverse transcription using Mg and heat or the option to fragment afterwards via tagmentation or sonication. Does anyone have any ideas for the best way to accomplish this?
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