View Single Post
Old 05-25-2019, 06:48 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238
Default

I would suggest to do a reaction according to standard protocol from start to end and check the resulting library. Tagmented DNA will not migrate according to size becuase:
1- transposon will be engaged with DNA and slow its migration speed
2- presence of salts in the buffer
nucacidhunter is offline   Reply With Quote