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Old 05-25-2019, 06:48 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

I would suggest to do a reaction according to standard protocol from start to end and check the resulting library. Tagmented DNA will not migrate according to size becuase:
1- transposon will be engaged with DNA and slow its migration speed
2- presence of salts in the buffer
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