SEQanswers - View Single Post - Low data output from questionable sequencing facility

itstrieu · 11-06-2019, 01:58 PM

Cluster density is kind of low for a v3 kit even for low diversity libraries. We usually sequence the V3V4 region on a V3 600 cycle kit and get around 50M PE reads on ~1000 K/mm2 with around 17 pM loading concentration.

11-06-2019, 01:58 PM	#10
itstrieu Member Location: Atlanta Join Date: Nov 2018 Posts: 27	Cluster density is kind of low for a v3 kit even for low diversity libraries. We usually sequence the V3V4 region on a V3 600 cycle kit and get around 50M PE reads on ~1000 K/mm2 with around 17 pM loading concentration.