SEQanswers - View Single Post - Low data output from questionable sequencing facility

samd · 11-06-2019, 02:27 PM

Hi SNPsaurus,

Ok so after discussing with them the 11M reads simply referred to the forward reads. The 23M PF refers to the forward and reverse. I see a 40% undetermined reads metric. Which is a bit high compared to 25% on my last run so I wonder if this is an indexing issue vs a sequencing issue?

@itstrieu: Damn I wish I could get those numbers. Would you recommend upping my concentration to 17pM instead of 13? Or is that dependent on other factors.

11-06-2019, 02:27 PM	#11
samd Member Location: California Join Date: Nov 2019 Posts: 20	Hi SNPsaurus, Ok so after discussing with them the 11M reads simply referred to the forward reads. The 23M PF refers to the forward and reverse. I see a 40% undetermined reads metric. Which is a bit high compared to 25% on my last run so I wonder if this is an indexing issue vs a sequencing issue? @itstrieu: Damn I wish I could get those numbers. Would you recommend upping my concentration to 17pM instead of 13? Or is that dependent on other factors.