SEQanswers - View Single Post - Low data output from questionable sequencing facility

itstrieu · 11-06-2019, 03:00 PM

You can increase cluster density by increasing the loading concentration.

In my post they are paired end reads so double that if you where doing single end reads. Here is a link how many reads you should expect for various MiSeq kits but it is dependent on cluster density. https://www.illumina.com/systems/seq...fications.html

Edit. Sometimes you hear the word cluster and reads used together. I believe that for a v3 kit, it can generate around 25M unique cluster and each cluster can do two reads for paired end so it would output 50 M reads

11-06-2019, 03:00 PM	#16
itstrieu Member Location: Atlanta Join Date: Nov 2018 Posts: 27	You can increase cluster density by increasing the loading concentration. In my post they are paired end reads so double that if you where doing single end reads. Here is a link how many reads you should expect for various MiSeq kits but it is dependent on cluster density. https://www.illumina.com/systems/seq...fications.html Edit. Sometimes you hear the word cluster and reads used together. I believe that for a v3 kit, it can generate around 25M unique cluster and each cluster can do two reads for paired end so it would output 50 M reads Last edited by itstrieu; 11-06-2019 at 03:06 PM.