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Old 11-06-2019, 04:21 PM   #17
Location: California

Join Date: Nov 2019
Posts: 20

Ok so I am guessing the "25M total reads" on Basespace actually means 50M since I did PE. Thanks for the suggestion I will look into that.

One thing I just remembered is the QC results were quite different from the first "good run" and the subsequent "bad runs". The good run has a nice skinny peak and the bad runs have lumpy peaks which I guess would be attributed to non-specific binding of primers? I've attached to the post in case anyone is interested or has any insight into that.
Again, thanks for all the feedback!
samd is offline   Reply With Quote