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Old 11-06-2019, 04:21 PM   #17
samd
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Location: California

Join Date: Nov 2019
Posts: 20
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Ok so I am guessing the "25M total reads" on Basespace actually means 50M since I did PE. Thanks for the suggestion I will look into that.

One thing I just remembered is the QC results were quite different from the first "good run" and the subsequent "bad runs". The good run has a nice skinny peak and the bad runs have lumpy peaks which I guess would be attributed to non-specific binding of primers? I've attached to the post in case anyone is interested or has any insight into that.
Again, thanks for all the feedback!
Sam
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