SEQanswers - View Single Post - Paired end seq read lengths are different

nucacidhunter · 08-21-2020, 08:51 PM

There is no technical constraint on the length of Illumina paired-end reads apart from R1 which should be 25 cycles. In some applications using asymmetric read length is more cost effective. For instance, 10x Genomics single cell RNA-seq libraries can be sequenced in 2x100 configuration using 200 cycle sequencing kit but it can be sequenced 28 cycles for R1 and 90 cycles for R2 using 100 cycle kit with identical outcome.

08-21-2020, 08:51 PM	#3
nucacidhunter Jafar Jabbari Location: Melbourne Join Date: Jan 2013 Posts: 1,238	There is no technical constraint on the length of Illumina paired-end reads apart from R1 which should be 25 cycles. In some applications using asymmetric read length is more cost effective. For instance, 10x Genomics single cell RNA-seq libraries can be sequenced in 2x100 configuration using 200 cycle sequencing kit but it can be sequenced 28 cycles for R1 and 90 cycles for R2 using 100 cycle kit with identical outcome.