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Old 08-21-2020, 08:51 PM   #3
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,238

There is no technical constraint on the length of Illumina paired-end reads apart from R1 which should be 25 cycles. In some applications using asymmetric read length is more cost effective. For instance, 10x Genomics single cell RNA-seq libraries can be sequenced in 2x100 configuration using 200 cycle sequencing kit but it can be sequenced 28 cycles for R1 and 90 cycles for R2 using 100 cycle kit with identical outcome.
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