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Old 09-16-2020, 02:55 PM   #6
cmbetts
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Location: Bay Area

Join Date: Jun 2012
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You might try to look at some of the method/kit comparison papers, or a paper describing a new library prep method. They often compare inter and intra assay performance of replicate inputs, usually Universal Human Reference RNA.

You'll likely only find older papers using technical replicates. The outcome of the early characterization experiments was that technical replicates are unnecessary, especially in comparison to the added value of biological replicates. They're essentially an elaborate way to burn money, but I'm sure Illumina would love you if you find an argument for them...
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