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Old 10-05-2020, 01:33 PM   #1
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Location: Florida, US

Join Date: Oct 2020
Posts: 2
Default NGS library enrichment question

I have a Hi-C library with P5 and P7 adapters. I would like to enrich for fragments containing a locus-of-interest by capture and extension with a biotinylated bait primer, and then amplification with a nested primer and either of the P5/P7 adapters.

Is is practical to add another sequencing primer adapter to the nested primer, so that I can have the new, enriched library paired-end sequenced? Or will I realistically have to clone the products from the enriched library?

I apologize for being new to NGS, and thanks in advance for any advice!
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