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Old 11-16-2010, 04:00 PM   #1
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Location: Saskatchewan

Join Date: Feb 2009
Posts: 6
Default Biological replicates for RNA-seq

Hi all,

I'm not sure if this has already been discussed elsewhere, but after looking around I didn't find anything directly answering my question, so if it has already been discussed, sorry for the repeat and please point me in the right direction!

I'm going to be doing RNA-seq for DE analysis of a bacteria growing in two different environments. I'm trying to determine the number of biological replicates that would be required to provide statistically-meaningful results. I saw that technical replicates aren't exactly necessary, and with cost of course being an issue, we were hoping to run 2 biological replicates of each environment, but we don't want to find out afterwards that we should have included more. We will be multiplexing our data, and are using illumina technology.

I'm a microbiologist, with little background in stats, so any input or thoughts on this would be greatly appreciated!

Thanks in advance.

- Vanessa
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