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Old 11-19-2010, 09:18 AM   #1
Location: Europe

Join Date: Nov 2009
Posts: 21
Default Can Ion Torrent be useful for microRNA sequencing?

Dear colleagues,
what do you think about applicability of Ion Torrent for miRNA profiling? They promise "Thousands to Millions of moderate reads per sample". "Moderate reads" is even excessive here, we need just short reads; my question is rather - if we have enough reads for adequate analysis. We have about thousand miRNAs (to be simple, I will not count precursors etc.), but some of them will be much more abundant then others. On my experience of real-time detection, I had Ct difference between highest and lowest about 11..13, i.e. concentration difference about 2000..8000 times, so I am wondering how many of less abundant miRNA species will be detected with this dynamic range.
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