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Old 12-10-2010, 09:18 AM   #3
Location: Boston, MA

Join Date: Oct 2009
Posts: 15

My primer concentration varied depending on the concentration of the ChIP DNA. For example, Illumina recommends 25uM primers for 10ng of DNA. If my sample was 1ng I would dilute the primers 10 fold. This is something I routinely do.

I was concerned about my qubit readings for the ChIP samples (seemed lower then normal). If that reading was off then because I dilute the adapters and primers those would've been off as well... but not sure that would've caused these double peaks.
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