View Single Post
Old 06-29-2010, 10:07 PM   #2
Senior Member
Location: The University of Melbourne, AUSTRALIA

Join Date: Apr 2008
Posts: 275

Originally Posted by nvteja View Post
Can anyone let me know why is it the case that the read quality is generally lower towards 3' end for solexa ?
Put simply, the reads are synthesised base by base from the 5' end. Each step is error prone, hence errors accumulate. Each step makes the molecule longer and wobblier, making it hard to do image processing.
Torst is offline   Reply With Quote