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  • Gene ordered by ID using getGenes(), package CummeRbund

    I want to produce a heat-map using a costumed gene list (accession IDs, e.g AT1G73590). I made a list of genes ordered depending on the processes they are involve in. I would like to conserve this order when I am plotting the expression value in csHeatmap(), but they end up ordered alphabetically.

    Code:
    Cand_GenesIds= as.character(Gene_name_ID[,3])
    head(Cand_GenesIds)
    [1] "AT1G19850" "AT1G73590" "AT4G32880" "AT5G16560" "AT1G32240" "AT4G17695"
    Then I recover the FPKM from cuffdiff using getGenes

    Code:
    Can_Genes<-getGenes(cuff, Cand_GenesIds)
    I plot the expression data using csHeatmap()

    Code:
    can.rep<-csHeatmap(Can_Genes,cluster="none",replicates=F, fullnames=F)
    can.rep
    Click image for larger version

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    The genes are ordered alphabetically and did not keep the order my list. Any thought?

  • #2
    I am having the same exact problem. Trying to view ~100 genes in a specific order. Anyone have any idea how to do this?

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