Hi,
I did a RNA-seq sample on Illumina GA2x: 15 years old Tumor FFPE sample. The RNA extraction give a very low RIN score analysing on the BioAnalyser RIN +/-1.5.
I used the EPICENTRE combination of RIBO-ZERO with SCRIPTSEQ v2. Good results 30-50% mapped reads, but still 35% related to rRNA. Is there a protocol to remove more rRNA out of the sample. Doing the Depletion twice, combinations Ribo-Zero with RiboMinus or other manors to increase the mapped reads?
thanks
c
I did a RNA-seq sample on Illumina GA2x: 15 years old Tumor FFPE sample. The RNA extraction give a very low RIN score analysing on the BioAnalyser RIN +/-1.5.
I used the EPICENTRE combination of RIBO-ZERO with SCRIPTSEQ v2. Good results 30-50% mapped reads, but still 35% related to rRNA. Is there a protocol to remove more rRNA out of the sample. Doing the Depletion twice, combinations Ribo-Zero with RiboMinus or other manors to increase the mapped reads?
thanks
c
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