Our core facility has many people (with very little money) interested in doing metagenomics on the MiSeq, but they often have different regions of interest. So we're looking for a different way to approach library prep other than the Caporaso approach of using PCR primers that include primers for the region of interest, indices, and Illumina adaptor sequences. This way seems to work well, but is very cost prohibitive since you need to order a separate reverse primer for every sample you want to multiplex. If you only want to do this once, you are paying ~$200 or more per sample for primers. For a set of 20 samples this can add up!
Has anyone seen or used a method that just ligates the Illumina adaptors/indices onto PCR amplicons? This would allow us to place the problem of actually amplifying the DNA onto our customers (and this is a big deal!) and should drastically save on the cost of library prep for these types of samples.
Thanks!
Has anyone seen or used a method that just ligates the Illumina adaptors/indices onto PCR amplicons? This would allow us to place the problem of actually amplifying the DNA onto our customers (and this is a big deal!) and should drastically save on the cost of library prep for these types of samples.
Thanks!
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