I want to perform a gene ontology analysis on a list of significant genes using the GO numbers directly and not the sequences/gene IDs.
I started using Blast2GO but the program first takes all of your sequences through NCBI BLAST, which is a very time consuming process. This seemed unnecessary to me because I already know my gene ID's. I decided to pull GO numbers directly from an online database, and successfully achieved this in 1 day as opposed to 7+...
I now have gene ontology information (GO:0005515, GO:0009540, etc.) for several thousand genes but cannot find a tool to analyze the meaning & distribution of these numbers directly. It shouldn't be too hard because all I have really done is skipped the first, and most time intensive, part of the Blast2GO process.
Something that could provide graphical output would obviously be ideal but it's not absolutely necessary.
Any help is much appreciated!
I started using Blast2GO but the program first takes all of your sequences through NCBI BLAST, which is a very time consuming process. This seemed unnecessary to me because I already know my gene ID's. I decided to pull GO numbers directly from an online database, and successfully achieved this in 1 day as opposed to 7+...
I now have gene ontology information (GO:0005515, GO:0009540, etc.) for several thousand genes but cannot find a tool to analyze the meaning & distribution of these numbers directly. It shouldn't be too hard because all I have really done is skipped the first, and most time intensive, part of the Blast2GO process.
Something that could provide graphical output would obviously be ideal but it's not absolutely necessary.
Any help is much appreciated!
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